Journal: PLOS Genetics
Article Title: A new regulator of the Staphylococcus aureus peptidoglycan hydrolase Sle1
doi: 10.1371/journal.pgen.1011990
Figure Lengend Snippet: (A) . Structured Illumination Microscopy (SIM) images of 8325-4 CxaR-GFP expressing a fluorescent derivative of CxaR from cxaR native chromosomal locus as the sole source of CxaR (left), labelled with fluorescent D-amino acid HADA which is incorporated into the cell wall (middle) and overlay of the two channels (right; CxaR-GFP signal in cyan, cell wall signal in magenta), showing late septal localization of CxaR-GFP. (B-D) SIM images showing CxaR-GFP localization upon deletion of ftsK (B) , deletion of clpX (C) or inactivation of ClpX (D) . CxaR-GFP septal localization is lost in the absence of FtsK or ClpX, while the protein accumulates in foci upon inactivation of ClpX. Representative images from 3 biological replicates. Scale bars 1 μm.
Article Snippet: Cells were harvested by centrifugation at 10000 rpm for 1 minute, resuspended in 20 μL of PBS and 1μL of this suspension was mounted on a microscopy slide with a 1.2% agarose (TopVision, Thermo Fisher Scientific) in PBS pad.
Techniques: Microscopy, Expressing